Group A Streptococcus

Group A Streptococcus

Methods and Protocols

;

Springer-Verlag New York Inc.

05/2020

432

Dura

Inglês

9781071604663

Pré-lançamento - envio 15 a 20 dias após a sua edição

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Part I: Genotyping and Genetic Manipulation of Group A Streptococcus 1. Detection of Streptococcus pyogenes Virulence Factors Aleksandra Kozinska and Izabela Sitkiewicz 2. Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens Nour Zahi Gammoh and Lothar Rink 3. The emm-cluster Typing System Pierre R. Smeesters and Anne Botteaux 4. Protocols for Tn-seq Analyses in The Group A Streptococcus Yoann Le Breton, Ashton T. Belew, and Kevin S. McIver 5. Genetic Manipulation of Group A Streptococcus - Gene Deletion by Allelic Replacement Timothy C. Barnett, Jessica N. Daw, Mark J. Walker, and Stephan Brouwer 6. Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging Jacelyn M. S. Loh, Kar Yan Soh, and Thomas Proft Part II: Application of Group A Streptococcus Omics 7. Whole Genome Sequence Analysis and Population Genomics of Group A streptococci Jake A Lacey, Taylah B James, Steven YC Tong, and Mark R Davies 8. Group A Streptococcus Transcriptome Analysis Misu Sanson and Anthony R. Flores 9. Protocol for Proteome Analysis of Group A Streptococcus Laura Wilk 10. Investigation of Group A Streptococcal Interactions with Host Glycan Structures using High Throughput Techniques: Glycan Microarray Analysis using Recombinant Protein and Whole Cells Anuk Indraratna, David De Oliveira, Lauren E Hartley -Tassell, Christopher J Day, Mark J Walker, Michael P Jennings, and Martina L Sanderson-Smith Part III: Assays to Investigate Group A Streptococcus Virulence Factors And Virulence Mechanisms 11. Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins Catherine (Jia-Yun) Tsai, Jacelyn M.S. Loh, Sam Blanchett, and Thomas Proft 12. Expression and Purification of Collagen-like Proteins of Group A Streptococcus Slawomir Lukomski and Dudley H. McNitt 13. Detection of Fibronectin-binding Proteins of Streptococcus pyogenes using Ligand Blot Analysis Masanobu Nakata and Shigetada Kawabata 14. Morphology and Ultrastructure of Group A Streptococcus Biofilms Tatyana A. Danilova, T.A. Smirnova, G.A. Danilina, A.A. Adzhieva, N.V. Shevlyagina, and V.G. Zhukhovitsky 15. Dynamic Interactions of Group A Streptococcus with Host Macrophages Angelica Riestra and J. Andres Valderrama 16. Identification of Group A Streptococcus-containing autophagosome-like vacuoles Takashi Nozawa and Ichiro Nakagawa 17. Flow Cytometry-based Assays to Quantify Complement Deposition and Neutrophil Uptake of Group a Streptococcus Nicola N.Lynskey 18. Live Cell Microscopy and Flow Cytometry to study Streptolysin S Mediated Erythrocyte Hemolysis Dustin L. Higashi, Charles R. Tessier, and Shaun W. Lee 19. Isolation of Monoclonal Antibodies to Group A Streptococcus Antigens Using Phage Display Jeremy M. Raynes, Mei Lin Tay, Sok H. By, John D. Steemson, and Nicole J. Moreland Part IV: Group A Streptococcus Killing Assays and Infection Models 20. Assays to Analyse Adhesion of Group A Streptococcus to Host Cells Adrina H. J. Khemlani, Thomas Proft, and Jacelyn M.S. Loh 21. The use of Galleria mellonella (wax moth) as an Infection Model for Group A Streptococcus Catherine (Jia-Yun) Tsai, Jacelyn M.S. Loh, and Thomas Proft 22. A Superficial Skin Scarification Method in Mice to Mimic Streptococcus pyogenes Skin Infection in Humans Manisha Pandey and Michael F Good 23. A Mouse Nasopharyngeal Colonisation Model for Group A Streptococcus Adrina H. J. Khemlani, Thomas Proft, and Jacelyn M. S. Loh 24. Humanised Plasminogen Mouse Model to Study Group A Streptococcus Invasive Disease Tania Rivera-Hernandez and Mark J. Walker 25. Lacefield Whole Blood Killing Assay to Evaluate Vaccine Efficacy Mark Reglinski 26. An Opsonophagocytic Killing Assay for the Evaluation of Group A Streptococcus Vaccine Antisera Reuben McGregor , Scott Jones , Jeremy Raynes , David Goldblatt , and Nicole J. Moreland Part V: Group A Streptococcus Therapies 27. Validation of Suitable Carrier Molecules and Target Genes for antisense Therapy using Peptide-coupled Peptide Nucleic Acids (PNAs) in Streptococci Gina Barkowsky, Bernd Kreikemeyer, and Nadja Patenge 28. Interfering with the Folding of Group A Streptococcal pili Proteins Fernanda Contreras and Jaime Andres Rivas-Pardo Part VI: Application of Group A Streptococcus-derived Methods 29. Use of Streptolysin O (SLO) to Study the Function of Lipid Rafts Heather E. Miller 30. Engineering of Group A Streptococcus Isopeptide Bonds into Immunoglobulin-like Protein Domains Paul G. Young and Edward N. Baker
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