ATM Kinase

ATM Kinase

Methods and Protocols

Kozlov, Sergei V.

Humana Press Inc.






15 a 20 dias

Descrição não disponível.
1. Assaying Radiosensitivity of Ataxia-Telangiectasia Hailiang Hu, Shareef Nahas, and Richard A. Gatti 2. Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology Amanda W. Kijas and Martin F. Lavin 3. ATM Gene Mutation Detection Techniques and Functional Analysis Guillaume Rieunier, Catherine Dubois D'Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern 4. An HTRF (R) Assay for the Protein Kinase ATM Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater 5. ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using CellomicsTM ArrayScan VTI Platform Catherine Bardelle and Joanna Boros 6. Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci Yi Chieh Lim 7. Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy Keiji Suzuki 8. Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla 9. Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike 10. Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov 11. Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS) Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo 12. Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies Sun Yingli and Du Fengxia 13. Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna 14. ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz 15. Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich 16. Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosphorylation Marina E. Borisova, Sebastian A. Wagner, and Petra Beli 17. Statistical Analysis of ATM-Dependent Signaling in Quantitative Mass Spectrometry Phosphoproteomics Ashley J. Waardenberg 18. ChIP Technique to Study Protein Dynamics at Defined DNA Double-Strand Breaks Jie Wen and Patrick Concannon 19. Studies of the DNA Damage Response by Using the Lac Operator/Repressor (LacO/LacR) Tethering System Rossana Piccinno, Marta Cipinska, and Vassilis Roukos 20. Imaging of Fluorescently-Tagged ATM Kinase at the Sites of DNA Double-Strand Breaks Anthony J. Davis, Shih-Ya Wang, David J. Chen, and Benjamin P.C. Chen 21. Live Cell Imaging to Study Real-Time ATM-Mediated Recruitment of DNA Repair Complexes to Sites of Ionizing Radiation-Induced DNA Damage Burkhard Jakob and Gisela Taucher Scholz 22. Analyzing Heterochromatic DNA Double Strand Break (DSB) Repair in Response to Ionizing Radiation Karolin Klement and Aaron A. Goodarzi 23. Phenotypic Analysis of ATM Protein Kinase in DNA Double-Strand Break Formation and Repair Elisabeth Mian and Lisa Wiesmuller 24. Monitoring DNA Repair Consequences of ATM Signaling Using Simultaneous Fluorescent Readouts Adrian Wiegmans 25. Non-Canonical ATM Activation and Signaling in Response to Transcription-Blocking DNA Damage Jurgen A. Marteijn, Wim Vermeulen, and Maria Tresini 26. Study of ATM Phosphorylation by Cdk5 in Neuronal Cells Hua She and Zixu Mao 27. DNA Damage Response in Human Stem Cells and Neural Descendants Jason M. Beckta, Bret R. Adams, and Kristoffer Valerie 28. A Patient-Specific Stem Cell Model to Investigate the Neurological Phenotype Observed in Ataxia-Telangiectasia Romal Stewart, Gautam Wali, Chris Perry, Martin Lavin, Francois Feron, Alan Mackay-Sim, and Ratneswary Sutharsan 29. Lentiviral Reprogramming of A-T Patient Fibroblasts to Induced Pluripotent Stem Cells Sam Nayler, Sergei Kozlov, Martin Lavin, and Ernst Wolvetang 30. Monitoring the ATM-Mediated DNA Damage Response in the Cerebellum Using Organotypic Cultures Efrat Tal and Yosef Shiloh
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